Steady State and Lifetime Nanotechnology EEM Spectrofluorometer

Our modular Nanolog Spectrofluorometer is specifically designed for research in nanotechnology and nanomaterials

The Nanolog® series of spectrofluorometers are specifically designed for research in nanotechnology and the frontiers of nanomaterials. A complete spectrum can be scanned as fast as a few milliseconds, and a full excitation emission matrix scan can be taken in just seconds.

Based on the world-wide proven technology of the Fluorolog®, the Nanolog detects fluorescence in the near-IR from 800 to 1700 nm (optional multi-channel detection to 2 µm, single-channel detection to 3 µm), with visible and UV options possible. With the Nanolog comes specially designed software called Nanosizer, ideal for classifying SWNTs, Quantum Dots and performing energy transfer calculations. Saving custom experimental routines and instrument layouts has never been easier.

Segment: Scientific

Manufacturing CompanyHORIBA Scientific


  • Rapid excitation-emission matrices in seconds
  • High sensitivity in near-IR with InGaAs array
  • High resolution
  • Eases qualification and quantification of species and families of SWNTs
  • Compatible with variety of detectors from UV to near-IR:
  • Photomultiplier tube for highest sensitivity and timeresolved analysis
  • Popular, cost-effective single-element InGaAsMulti-element CCD array for fast data-acquisition
  • Resolve mixtures of quantum dots simultaneously Perform energy-transfer experiments
  • Modular design for your ideal experimental setup


FluorEssence Software

  • Simplified drop-down menus
  • Detector-algebra for customized data acquisition
  • Matrix scanning for 3-D data
  • Real-time control for instant effects of changing the hardware
  • Contour maps and 3-D perspective plots
  • Curve-fits
  • Deconvolution
  • Smoothing
  • Excitation and emission correction
  • Derivatives and integration
  • Standard arithmetic


Nanosizer® Software

Nanosizer® – for Single‐Walled Carbon Nanotube Excitation‐Emission Map Simulation and Analysis

Nanosizer® in Origin® Pro 8 simplifies the process for simulation and analysis for single‐walled carbon nanotube excitation‐emission map simulation and analysis. Nanosizer is used with our Nanolog spectrofluorometers, which are specifically designed for research in nanotechnology and nanomaterials. Nanosizer comes with our patented double-convolution-integral algorithm specially designed for determining chirality and diameter of single-walled carbon nanotubes.

Nanosizer® lets you simulate excitation-emission maps of SWNT near-IR fluorescence to compare to your actual data. Using built-in or custom libraries, Nanosizer® rapidly assigns specific peaks to particular SWNT (n,m) structures, and even generates helical maps. Nanosizer® also greatly simplifies FRET studies of SWNT bundles, length-distribution analyses, and nanotube purification analyses. Nanosizer®even offers a platform suitable to support future ISO and ASTM standards for identification and purification of semiconducting SWNTs.

Perfect for FRET in SWNT Bundles, Length Distribution Analysis, and Purification applications

Features and Benefits of Nanosizer® in OriginPro® 8

  • 3-D spectral surface simulation
  • Simultaneous analytical simulation of spectral surfaces
  • Rapid preliminary scanning to recognize peaks and their shapes for easy model fitting
  • Complete, easy-to-edit model-parameter table for nanotube mixtures
  • Nanotube species recognition with editable library
  • Nanotube species recognition with user’s analytical simulations
  • Complete reports and charts in common spreadsheet format
  • Optional “enhanced” fitting-engines for statistically robust simulations

Nanolog Specifications

All-reflective optics Nanolog fluorescence spectrometer for perfect focus at all wavelengths from the UV to NIR

Excitation Sources Choice of:

  • 450 W xenon short-arc lamp housing with off axis ellipsoidal collector and optional pulsed xenon lamp enhancement (for time resolved phosphorescence)
  • 75 W xenon PowerArcTM lamp housing with enveloping ellipsoidal collector.

Note: The Sensitivity Specification listed below is the same with either the 450 W xenon or the 75 W xenon due to the enhanced collection efficiency of the PowerArc!

Excitation Monochromators Choice of:

  • 180 mm Czerny-Turner monochromator with kinematic gratings and all-reflective optics in either single or double

monochromator configuration (the 360 mm focal length double monochromator is recommended for the highest stray-light rejection and sensitivity).

  • iHR320 triple-grating turret 320 mm spectrometer with one or two entrance ports.

Note:Specifications hereare basedon 180mm monochromator withstandard 1200grooves/mm gratingblazed at 330nm. Othergratings areavailable.

Resolution: 0.2 nm
Accuracy 0.5 nm
Speed 150 nm/s
Range 0–1300 nm mechanical range; throughput based on grating’s blaze
Bandpass Set automatically (0–30 nm single-grating, 0–15 nm double-grating)
Sample Compartment All reflective optics sample compartment with single cuvette sample compartment tray for quick replacement with variety of optional sample hold- ers. Reference photodiode for excitation correction from 240–1000 nm. Optional front-face detection for highly turbid samples in solution. Optional T-Format detection to allow optional second emission-detection channel.
Emission Imaging Spectrograph iHR320, for multi-channel acquisition, with triple-grating turret. Can be configured with one or two exit ports each for multi-channel or single channel detectors. Equipped with 150 grooves/mm grating for multi-channel detection of entire emission spectra with a single acquisition.
Resolution 0.2 nm
Accuracy 0.3 nm
Range 0–1500 nm mechanical range (using a 1200 grooves/mm grating and single channel detector)
Multichannel Detectors Choice of up to two of the following:

  • SymphonyTM IGA, 512 pixels, 800 to 1,700 nm, LN-cooled
  • Symphony Extended IGA, 512 pixels, 1,100 to 2,200 nm, LN-cooled
  • SyncerityTM CCD, 1024 x 256 pixels, TE-cooled (-60oC)
  • Synapse PlusTM CCD, 1024 x 256 pixels, 200 to 1,100 nm, TE-cooled (-80 oC)
  • Symphony CCD, 1024 x 256 pixels, LN-cooled
Optional T-Side Emission Monochromator for Single Channel Detectors Specifications are the same as excitation monochromator above1
Single Channel Detectors Choice of:

  • Room temperature PMT housing with R928 (185 to 850 nm) or R13456 (185 to 950 nm)
  • TE-cooled PMT housing with R2658 (185 to 1,050 nm)
  • TE-cooled PMT housing with H10330-45 (950 to 1,400 nm) or H10330-75 (950 to 1,700 nm)
  • LN-cooled PMT housing with R5509-43 (300 to 1,400 nm) or R5509-73 (300 to 1,700 nm)
  • LN-cooled IGA (1.7) detector, 800 to 1,550 nm
  • LN-cooled extended IGA(1.9) detector, 1,000 to 1,750 nm
  • LN-cooled extended IGA(2.1) detector, 1,000 to 2,000 nm
  • LN-cooled extended IGA(2.6) detector, 1,000 to 2,400 nm
Software Windows™-based FluorEssence™ software supplies all scanning, time-based, and accessory data acquisition plus complete control of all hardware, plus Nanosizer™ for fitting of single-walled carbon nanotube spectra to known library to determine chiralities and diameters.
Sensitivity Water Raman Signal-to-Noise Ratio of 15,000:1 (FSD method), 350 nm excitation, 5 nm bandpass, 1 second integration, no filters or averaging, with R928P photomultiplier tube.


Recommended configurations

The Nanolog 3-22-iHR, pictured here, is configured with a double-grating excitation and emission monochromator, plus an imaging spectrograph for a second emission channel.

Schematic shows a highly versatile Nanolog equipped with a 450 watt xenon lamp housing, double excitation monochromator for ultimate stray light rejection, T-Format sample compartment with multichannel iHR320 imaging spectrograph equipped with a Symphony NIR InGaAs array detector and a T-Side double emission monochromator with a PMT detector for ultimate sensitivity and stray light rejection.

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