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DeltaFlex

TCSPC/MCS Fluorescence Lifetime System

Ultimate TCSPC/MCS Performance and Flexibility for Lifetimes From 5 Picoseconds to Seconds

The HORIBA DeltaFlex™ system is the Time-Correlated Single Photon Counting (TCSPC) system of choice for the measurement of fluorescence lifetimes from picoseconds to seconds. Taking advantage of more than 45 years of TCSPC innovation, it is a complete fluorescence/phosphorescence system designed and optimized with innovative pulsed lasers and LEDs, timing electronics, detectors and sample handling components, all powered by HORIBA’s highly intuitive and extremely powerful EzTime™ touchscreen software interface.

Segment: Scientific
Manufacturing CompanyHORIBA Scientific

 

Unique Benefits from a Lifetime in Fluorescence

  • Flexibility to meet any requirement
  • Better user experience with automated component recognition and control
  • Extensive choice of sources and detectors to meet all wavelength and lifetime requirements
  • EzTime intuitive, automated, touchscreen software

DeltaFlex: Flexibility is in the name, and flexibility is in the design

The modular DeltaFlex system is comprised of a choice of the following main components:

  • Optical configuration
    Choose from filters or monochromators and single detector (L format) or simultaneous dual detector (T Format)
  • Excitation sources
    Choose from a plethora of pulsed lasers and LED sources
  • Detection modules
    Choose from a wide range of dedicated TCSPC detectors depending on your emission wavelength range, lifetime requirements and budget
  • Timing electronics
    Choose from standard or high resolution electronic interfaces depending on your lifetime requirements

 

All DeltaFlex systems are controlled using EzTime software which provides for full instrumentation control and acquisition, as well as a comprehensive suite of data analysis modules for fluorescence and phosphorescence lifetime determination, decay associated spectra (DAS), time-resolved anisotropy and uncorrected steady state emission spectra (if equipped with a scanning emission monochromator).

Optional Pulsed Laser and LED Light Sources

DeltaDiode™ General Specifications

DeltaDiode Type Pulse Duration Repetition Rate Available Wavelengths
DeltaDiode Laser
(Denoted with an “L” on part number)
35 to 200 ps 10 kHz to 100 MHz 375 to 1,310 nm
DeltaDiode LED
(No “L” on part number)
750 to 950 ps 25 MHz 265 to 455 nm
scrollable

 

DeltaDiode Controller Specifications

DD-C1 Controller

Function Specification
Repetition Rates 10 kHz, 20 kHz, 50 kHz, 100 kHz, 250 kHz, 500 kHz, 1 MHz, 2 MHz, 4 MHz, 5 MHz, 8 MHz, 10 MHz, 16 MHz, 20 MHz, 25 MHz, 50 MHz, 80 MHz, 100 MHz, or Trigger Input (single-shot to 50 MHz). Subject to attached head

Trigger Input

Pulse amplitude +0.5 V to +5 V, trigger threshold software programmable from +0.2 V to +2 V, 50 Ω, 20 ns minimum spacing

Sync Outputs

Simultaneous output of NIM-compatible (-0.8 Vpp 50 Ω) and TTL-compatible (+2 Vpp 50 Ω), automatic width selection 4-15 ns nominal

Sync Delay Control

Adjustment of sync output pulse timing in range -10 ns to +10 ns nominal in 1 ns steps, uncalibrated

Fast Gate Input

Pulse amplitude +0.5 V to +5 V, trigger threshold software programmable from +0.2 V to +2 V, 50 Ω. Selectable Inhibit/Enable modes

Slow Gate Input

Pulse amplitude +2 V to +5 V. 10 kΩ. Selectable Inhibit/enable modes. Operates in Pulsed and CW modes

Interlock

2-pin connector (included). Contacts must be short-circuited to enable emission

Connection to Head

1.5 m cable (included)

User Interface

LCD display (stand-alone operation) or software (PC control)

PC interface

USB 2.0 with integral hub for downstream connection to other USB peripherals (cable to host PC and software supplied)

Power Requirement

90 V to 250 V AC, 50/60 Hz, 100 VA

Operating Temperature

+15˚ C to +30˚ C (ambient)

Weight & Dimensions

3.1 kg, 234 x 255 x 92 mm

scrollable

 

Solas MOFA Fiber Laser Specifications

Parameter

Solas 355L

Solas 532L

Solas 1064L

Power

> 7 mW at 80 MHz

> 30 mW at 80 MHz

> 300 mW at 80 MHz

Power Stability

0.5% RMS over 10 min

Pulse duration

80 to 100 ps (FWHM)

Spectral Width

< 0.2 nm

< 0.4 nm

Resolution Rate

Pulse on demand to 100 MHz

Pointing Stability

< 1 μrad

Polarization Extinction Ratio

> 20 dB

Beam Quality

Multi-mode fiber output

Circular TEM00 beam

scrollable

 

SpectraLED Specifications

Pulse Duration Repetition Rate Available Wavelengths
SpectraLED < 250 ns to > 10 s 0.1 to 100 kHz 265 to 1,275 nm
scrollable

 

SpectraXE Specifications

Pulse Duration Repetition Rate Available Wavelengths
SpectraXE 0.4 μs 0.1 Hz to 80 Hz 185 to 2,000 nm
scrollable

 

HPPD Specifications

Model Wavelength Range Temporal Response
(IRF FWHM)
Dark Count Quantum Efficiency
HPPD-650 220 to 650 nm 50 ps < 100 cps 28% (340 nm)
HPPD-720 300 to 720 nm 120 ps < 1000 cps 47% (530 nm)
HPPD-860 220 to 860 nm 50 ps < 200 cps 23% (280 nm)
HPPD-870 300 to 870 nm 130 ps < 500 cps 26% (630 nm)
HPPD-890 380 to 890 nm 160 ps < 1000 cps 16% (630 nm)
scrollable

 

MCP-PMT Detectors

HPPD-860 COOLED MCP-PMT (R3809-50)
Typical IRF FWHM at 400 nm 40-45 ps 40-45 ps
Shortest Measurable Lifetime 40-45 ps 5 ps
Wavelength Response  220-860 nm 160-850 nm
Robust for Steady State Spectra Yes No
Amplifier + CFD Integrated (no cable) External
High Voltage Bias Integrated (no cable) External
Compatible with Phos and Steady State Yes No (requires second detector)
Temperature Control Integrated TEC (air-cooled) External (water-cooled)
Dark Count Rate (Cooled)  < 200 cps  < 20 cps
PC Interface N/A N/A
scrollable

 

NIR TCSPC Specifications

 

Model Sensor Wavelength Range Temporal Response Dark Count Cooling
NIR-R4 R5509-43 PMT 300 to 1,400 nm 1.5 ns (TTS) < 25,000 cps Liquid nitrogen
NIR-R7 R5509-73 PMT 300 to 1,700 nm 1.5 ns (TTS) < 250,000 cps Liquid nitrogen
NIR-H2 H10330-25 PMT 950 to 1,200 nm 400 ps (TTS) < 2,500 cps Thermo-electric
NIR-H4 H10330-45 PMT 950 to 1,400 nm 400 ps (TTS) < 25,000 cps Thermo-electric
NIR-H7 H10330-75 PMT 950 to 1,700 nm 400 ps (TTS) < 250,000 cps Thermo-electric
NIR-S1 Count-100N SPAD 400 to 1,000 nm < 3 ns (TTS) 100 cps None
scrollable

 

FiPho TCSPC Electronics Specifications

Specifications

FiPho

FiPho-HR

Full Detectable TCSPC Lifetime Range

<20 ps to 30 sec

5 ps to 30 sec

TCSPC Converter Type

Digital TDC

Digital TDC and Analog TAC

TCSPC Bin Width

<15 ps

~ 250 fs

Phosphorescence Mode

MCS

MCS

Independent Stop Channels

1 to 4

1 to 4

Photon Streaming

Included

Included

FLIM Capable

Yes

Yes

scrollable

 

Detailed FiPho Electronics Specifications

Specifications

FiPho (TDC, MCS)

FiPho-HR (TDC, TAC, MCS)

Full Detectable Lifetime Range

<20 ps to 30 sec

5 ps to 30 sec

TCSPC Time Range

<2 ns to 55 μs

<2 ns to 55 μs

Deadtime

5 ns

5 ns

TCSPC Bin Width

<15 ps

~ 250 fs

Electronics Jitter (FWHM)

30 ps

< 10 ps

TCSPC Histogram Size

Up to 16k

Up to 64k

Histogram Bin Depth

32 bit

32 bit

Independent Stops

1 to 4

1 to 4

Maximum Start Rate

100 MHz

100 MHz

Maximum Stop Rate

40 Mcps

40 Mcps

Operating Mode

Automatic Forward Timing

Automatic Forward or Reverse timing

Streaming Mode

Photon Streaming (Time-Tag)

Photon Streaming (Time-Tag)

MCS Bin Width

5 ns

5 ns

MCS Time Range

< 2.5 μs to 330 seconds

< 2.5 μs to 330 seconds

Maximum MCS Histogram Size

64k

64k

Acquisition and Analysis Macro Scripting

Yes

Yes

PC Interface

USB 3.0

USB 3.0

Software

EzTime, EzTime Image

EzTime, EzTime Image

scrollable

HORIBA Scientific has a policy of continuous product development, and reserves the right to amend part numbers, descriptions and specifications without prior notice.

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